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DE111 Bacillus subtilis MTCC 5856

Also known as: Bacillus subtilis DE111, DE111, DE111®

Overview

Bacillus subtilis DE111 is a well-researched, spore-forming probiotic bacterium widely used as a dietary supplement. Unlike many other probiotics, its spore-forming nature allows it to survive harsh gastric conditions, ensuring its delivery to the small intestine where it germinates and exerts its beneficial effects. It is primarily recognized for its ability to support gut microbiome health, modulate immune function, and potentially improve gastrointestinal comfort. While often discussed alongside other Bacillus strains like Bacillus coagulans MTCC 5856, it is crucial to note that DE111 is a distinct strain with its own unique properties and research profile. Its high thermostability makes it suitable for various supplement formulations. Research indicates that DE111 contributes to a balanced gut environment and supports the body's natural defenses, making it a valuable addition to probiotic regimens for healthy adults.

Benefits

Bacillus subtilis DE111 offers several evidence-based benefits, primarily centered on gut health and immune modulation. In a randomized, double-blind, placebo-controlled trial involving 46 healthy adults, daily supplementation with 1 × 10^9 CFU of DE111 for four weeks demonstrated immunomodulatory effects, suggesting its role in supporting a healthy immune response. While specific quantitative outcomes for immune modulation were not detailed, the study indicates a positive impact on the immune system. Furthermore, DE111 is known to support gut barrier function and promote diversity within the gut microbiome, contributing to overall digestive health. These benefits are particularly relevant for healthy adults and individuals experiencing mild gastrointestinal discomfort. The spore-forming nature of DE111 ensures its survival through the digestive tract, allowing it to effectively colonize and exert its effects. Benefits are typically observed within four weeks of consistent supplementation.

How it works

Bacillus subtilis DE111 operates through several key mechanisms to exert its probiotic effects. As a spore-forming bacterium, its spores are highly resistant to the acidic environment of the stomach, allowing them to survive gastric transit and reach the small intestine intact. Once in the small intestine, these spores germinate into active vegetative cells. These active cells then transiently colonize the gut, where they contribute to a balanced microbial environment by promoting the growth of beneficial bacteria and potentially inhibiting the proliferation of undesirable ones. DE111 also modulates the immune response, interacting with immune cells in the gut-associated lymphoid tissue (GALT) to support systemic immunity. Additionally, it helps maintain the integrity of the gut barrier by supporting tight junctions between epithelial cells, which is crucial for preventing the leakage of toxins and undigested food particles into the bloodstream.

Side effects

Bacillus subtilis DE111 is generally recognized as safe (GRAS) for healthy adults, with a favorable safety profile. Clinical trials have reported rare instances of mild gastrointestinal discomfort, such as bloating or gas, in a minority of individuals, but these are typically transient and mild. Uncommon or severe side effects have not been commonly reported in available studies. There are no significant drug interactions identified with DE111, making it compatible with most medications. Contraindications are not established for healthy adults. However, caution is advised when considering its use in immunocompromised individuals due to limited data in this specific population. As with any supplement, it is recommended to consult a healthcare professional before use, especially for individuals with underlying health conditions or those taking other medications. Overall, DE111 is considered a safe and well-tolerated probiotic when used as directed.

Dosage

The recommended dosage for Bacillus subtilis DE111 typically ranges from 1 to 5 × 10^9 CFU (colony-forming units) per day. The minimum effective dose observed in studies is 1 × 10^9 CFU/day. Doses up to 5 × 10^9 CFU/day have been safely used in clinical settings, and a maximum safe dose has not been formally established, indicating a wide margin of safety. DE111 can be taken once daily, with or without food, as its spore-forming nature ensures survival through gastric acid regardless of meal timing. It is commonly available in capsule or powder forms, and its spores are highly stable, maintaining viability in various matrices. No specific cofactors are required for its absorption or efficacy, as the spores are designed to survive and germinate independently in the small intestine.

FAQs

Is Bacillus subtilis DE111 safe for daily use?

Yes, Bacillus subtilis DE111 is generally recognized as safe (GRAS) for healthy adults and can be used daily. Clinical trials have reported no serious adverse events.

When is the best time to take DE111?

DE111 can be taken at any time of day, with or without food. Its spore-forming nature ensures its survival through stomach acid, making timing flexible.

What are the expected benefits of taking DE111?

Expected benefits include improved gut comfort, support for a balanced gut microbiome, and modulation of the immune system. Benefits are typically observed within 4 weeks.

Is DE111 the same as other Bacillus strains like MTCC 5856?

No, Bacillus subtilis DE111 is a distinct strain from Bacillus coagulans MTCC 5856. While both are probiotics, they have different properties and research profiles.

Research Sources

https://pmc.ncbi.nlm.nih.gov/articles/PMC7957723/ – This randomized, double-blind, placebo-controlled trial investigated the immunomodulatory effects of Bacillus subtilis DE111 (1 × 10^9 CFU/day) in 46 healthy adults over 4 weeks. The study found that DE111 supplementation was associated with immunomodulatory effects compared to placebo, suggesting its potential role in supporting immune function in healthy individuals.
https://pmc.ncbi.nlm.nih.gov/articles/PMC8366289/ – This mechanistic and clinical study, involving ileostomy patients, demonstrated that Bacillus subtilis DE111 spores successfully survive gastric transit and germinate in the small intestine within three hours. This research provides strong evidence for the viability and effective delivery of DE111 to the gut, supporting its probiotic mechanism of action.
https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1101144/full – This review discusses the characteristics of Bacillus subtilis DE111, highlighting its spore-forming nature, high thermostability, and ability to survive gastric transit. It summarizes the current understanding of DE111's mechanisms of action, including its role in supporting gut barrier function, modulating the immune system, and promoting beneficial microbial populations.
https://foodandnutritionresearch.net/index.php/fnr/article/view/1218 – This clinical trial, though focused on Bacillus coagulans MTCC 5856, is included for comparative context within the probiotic class. It reported significant improvements in depression and IBS quality of life scores, along with reductions in inflammatory biomarkers, demonstrating the potential of spore-forming probiotics for gut-brain axis benefits.